Enzyme Sucrase + β-galactosidase

EC Number:    
- Sucrase: 3.2.1.20,
- β-galactosidase: 3.2.1.2

CAZy Family: GH13, GH35

CAS Number:  
- Sucrase: 9001-42-7
- β-galactosidase: 9031-11-2

Synonyms:
- Sucrase: α-glucosidase; α-D-glucoside glucohydrolase,
- β-galactosidase: β-galactosidase; β-D-galactoside galactohydrolase

Source:
- Sucrase: Bacillus amyloliquefaciens
- β-galactosidase: Aspergillus niger

Molecular weight: 57,750

Concentration: Sucrase (170 U) / β-galactosidase (3,000 U)

Specificity:
This enzyme mixture gives complete hydrolysis of sucrose, maltose and lactose under the defined assay conditions, with no hydrolysis of the trisaccharide, β-D-Fruf-(2,1)-β-D-Fruf-(2,1)-β-D-Fruf

Specific activity: Sucrase (170 U) / β-galactosidase (3,000 U)

Unit definition: 
- Sucrase: One unit of sucrase activity is defined as the amount of enzyme required to release one µmole of glucose per minute from sucrose (10 mM) in sodium phosphate buffer (100 mM), pH 6,8 at 40°C
- β-galactosidase: One Unit of β-galactosidase activity is defined as the amount of enzyme required to release one µmole of p-nitrophenol (pNP) per minute from p-nitrophenyl-β-D-galactoside (10 mM) in sodium acetate buffer (100 mM), pH 4,5 at 40°C

Temperature optima: 40°C
pH optima: 5

Stability: minimum 1 year at < -10°C

• High purity and stability

- Research, biochemical enzyme assays and in vitro diagnostic analysis
- Used for removal of sucrose, maltose and lactose in the measurement of dietary fibre

Data Booklet Safety data sheet

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